Laboratory # 5: Paper Chromatography of Food Dyes as well as Ink

Esther Kadoch


Provided to

Roger Machaalani

Dawson University



March 24th, 2021

March 31th, 2021

Information Sheet:

Make-up of the mobile stage: 0.1% (m/v) NaCl remedy

Range of solvent front from beginning (centimeters): 6.90-7.30 centimeters

If the color divided right into even more than [you might require to separate some of the boxes right into several rows [/h3>

one colour per place. Usage much less or include additional rows as essential.

Table 1: Determined Rf worths and also recognition of areas on chromatogram(s)

Area number Place Tag



Rf Worth FD&C Dye #

1 Red

Red 40 Red 3

2 Eco-friendly

Yellow 5 Blue 1

3 Blue 7.05 7.05 1.

Blue 1Red 40

4 Yellow 4.70 7.20 0.

Yellow 5 Red 40



Yellow 6 Red 2Blue 1



Red 3Blue 1



5.20 7.30 0.71 Yellow 6

Speculative Configuration:


1. With the recognized FD&C dyes you recognized in the business food colourings, were any one of

the exact same FD&C dyes discovered in the food/item examples you examined?

Yes, in the purple as well as brownish Crayola Pen blue 1 is discovered, which are discovered in the environment-friendly

and also blue food colouring from the Club Home routine pack.

2. In preparing the chromatography paper, a beginning line is pulled in pencil for the placing

of the areas. Why would certainly a 'wet-erase' (non-permanent) not be suitable for attracting the beginning in

this experiment?

It is not suitable to make use of to attract the beginnings line due to the fact that, like in the instance given up the laboratory

record, it would certainly diffuse throughout the chromatography paper and also would certainly hinder the information

of the various other places.

3. Why did some ink dyes take a trip to the top of the paper as well as various other ink dyes continued to be near the

base of the paper? Discuss in regards to the destinations of the fragments for either the mobile

fluid stage or the strong paper fixed stage.

The reduced the Rf worth (with the tiniest range passed by the substance), the much less

Since it is extra drawn in to the paper than to the solute, polar the particle is. Because the

remedy is polar, the substances that are brought in to the solute (common salt) have a greater

polarity, and also have greater Rf worths.